Zebrafish Oocyte Maturation Bioassay As a Screening Tool for Selected Environmental Chemicals
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
Date of Award
Summer 2016
Abstract
Certain environmental chemicals are capable of acting as hormonal mimics or receptor blockers and may permanently modify the development of the reproductive system. Seven chemical treatments: a progestin hormone 17α, 20β-Dihydroxy-4-pregnen-3-one (DHP), and six environmental chemicals (genistein, endosulfan, malathion, iprodione, carbaryl, and glyphosate) were selected for screening. The number of oocytes undergoing maturation, i.e., resumption of meiosis after prophase I arrest, was determined by visualization of germinal vesicle (nucleus) breakdown (GVBD). The clear oocytes undergoing GVBD were counted and expressed as a percentage of the total oocytes exposed.
Advisor
Izhar A Khan
Subject Categories
Biochemistry, Biophysics, and Structural Biology | Biology | Life Sciences
Recommended Citation
Maskey, Era, "Zebrafish Oocyte Maturation Bioassay As a Screening Tool for Selected Environmental Chemicals" (2016). Electronic Theses & Dissertations. 808.
https://digitalcommons.tamuc.edu/etd/808
