Title

Zebrafish Oocyte Maturation Bioassay As a Screening Tool for Selected Environmental Chemicals

Author

Era Maskey

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Date of Award

Summer 2016

Abstract

Certain environmental chemicals are capable of acting as hormonal mimics or receptor blockers and may permanently modify the development of the reproductive system. Seven chemical treatments: a progestin hormone 17α, 20β-Dihydroxy-4-pregnen-3-one (DHP), and six environmental chemicals (genistein, endosulfan, malathion, iprodione, carbaryl, and glyphosate) were selected for screening. The number of oocytes undergoing maturation, i.e., resumption of meiosis after prophase I arrest, was determined by visualization of germinal vesicle (nucleus) breakdown (GVBD). The clear oocytes undergoing GVBD were counted and expressed as a percentage of the total oocytes exposed.

Advisor

Izhar A Khan

Subject Categories

Biochemistry, Biophysics, and Structural Biology | Biology | Life Sciences

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